Inonotus obliquus Polysaccharides Inhibited Cellular Growth of NCI-H23 and A549 Lung Cancer Cells Through G0/G1 Cell Cycle Arrest and ROS Mediated Cell Death

Chaga mushroom (Inonotus obliquus) has been used for a long time as folk medicine treating multiple diseases in several parts of the world without rendering any undesired toxicity. In this study, I. obliquus polysaccharides (IOP) were extracted and assessed to determine their anti-tumorigenic potential human lung cancer cell lines NCI-H23 A549 using cytotoxicity apoptosis assays. MTT assay revealed significant reduction viability (p < 0.05) exposed IOP (5-200 µg/mL) concentration-dependent manner with IC50 100 µg/mL both lines. Cell 50 further analyzed. arrested growth at G0/G1 stage that can implicate an anti-proliferative effect cells. Intracellular reactive oxygen species (ROS) generation was detected DCFH-DA dye demonstrated increased levels ROS 0.05). Assessment mitochondrial membrane JC-1 exhibited decreased potential, characterized by low dye-intake, shown flow cytometry. addition, Annexin-V/FITC analysis cytometry significantly number apoptotic cells manner. These results showed induce tumor lines, therefore might be considered effective anti-tumor agent could exploited clinical settings.